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Literature summary extracted from

  • Francis, S.H.; Turko, I.V.; Grimes, K.A.; Corbin, J.D.
    Histidine-607 and histidine-643 provide important interactions for metal support of catalysis in phosphodiesterase-5 (2000), Biochemistry, 39, 9591-9596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.35 expression of PDE5 in Sf9 insect cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.35 D644A 10% and 4% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 D714A 33% and 22% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 D754A 59% and 28% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 E632A 20% and 16% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 E672A 3% and 8% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 H603A 38% and 22% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 H607A 3.9% and 13% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 H643A 3% and less than 1% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 H647A less than 1% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 H675A 9% and 4% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus
3.1.4.35 N604A less than 2% of maximal activity in the absence of Mn2+ and Mg2+, respectively Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.35 Mg2+ approx. 3fold increase in activity at 10 mM Bos taurus
3.1.4.35 Mn2+ approx. 5fold increase in activity at 10 mM Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O Bos taurus
-
 guanosine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.35 recombinant PDE5 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.35 lung
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 guanosine 3',5'-cyclic phosphate + H2O
-
 Bos taurus guanosine 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.35 cGMP-binding cGMP-specific PDE
-
 Bos taurus
3.1.4.35 PDE5
-
 Bos taurus